Development of Pyrosequencing Method for UGT1A1 Polymorphisms in Thai Colorectal Cancer
نویسندگان
چکیده
The objective of this study was to determine allele frequency and genotyping of UGT1A1 polymorphisms (UGT1A1*28 and UGT1A1*6) in Thai colorectal cancer patients who received irinotecan treatment and develop pyrosequencing technique for UGT1A1 genetic polymorphisms detection. The Ninety-one patients entered the study. The results showed the allele frequencies for UGT1A1 genetic polymorphisms were 54.95% (*1/*1), 13.19% (*1/*6), 25.27% (*1/*28), 4.40% (*28/*6) และ 2.20% (*28/*28). No homozygous mutation UGT1A1*6/*6 was found in our population. Genotyping by pyrosequencing were 100% concordant with capillary electrophoresis sequencing direct sequencing for validation. The technique we set up is appropriate for the detection of UGT1A1 genetic polymorphisms in routine practice before irinotecan therapy. And this technique is rapid, reliable, and more cost-effective. ค าส าคัญ: ยีน UGT1A1, ความหลากหลายทางพันธุกรรม, ไพโรซีเควนซิ่ง, ยาเออริโนทีแคน
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